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In ArcLight, this residue is mutated to a glutamine (R217Q), which places the midpoint of voltage sensor response at approximately -20 mV.

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Significance between every two groups was calculated using unpaired two-tailed Student’s t test. Two-way Anova was performed for comparison of two groups over time followed by Sidak’s multiple test. *P < 0.05, **P < 0.01; n = 10 per group.

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Gene promoters have guided evolution processes for millions of years. It seems that they were the main engine responsible for the integration of different mutations favorable for the environmental conditions.

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